β-胡萝卜素降解菌HC-3发酵条件优化及其对再造烟叶浓缩液增香效果研究
Optimization of fermentation conditions ofβ-carotenes degradating strain HC-3 and its effect on aroma enhancement of reconstituted tobacco concentrate
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摘要:运用单因素试验和正交试验，对 β-胡萝卜素降解菌株HC-3的发酵条件进行优化，并将最佳发酵条件下得到的菌株HC-3发酵液应用于再造烟叶浓缩液中以研究其增香效果.结果表明：菌株HC-3发酵降解 β-胡萝卜素的最佳条件为NaNO 3质量浓度3 g/L，蔗糖质量浓度30 g/L，酵母粉质量浓度3 g/L，初始pH值7.0，在该发酵条件下 β-胡萝卜素降解率可达93.35%.经菌株HC-3处理后，再造烟叶浓缩液中的二氢猕猴桃内酯、4，7，9-巨豆三烯-3-酮、4-羟基- β-二氢大马酮、9-羟基-4，7-巨豆三烯酮、柠檬酸三乙酯、4-（3-羟基丁基）-3，5，5-三甲基-2-环己烯-1-酮、肉豆蔻酸和新植二烯这8种香味成分含量明显提高；与未经处理的再造烟叶浓缩液相比，利用菌株HC-3处理后的再造烟叶浓缩液制得的片基香气质较好、香气量较足、香气浓度较高、杂气量较低.Abstract:The fermentation conditions of β-carotene degrading strain HC-3 were optimized by single factor experiment and orthogonal experiment,and the fermentation liquid of strain HC-3 obtained under the optimal fermentation conditions was applied to the reconstituted tobacco concentrate to study its aroma enhancing effect.The results showed that the best fermentation conditions for β-carotene degradation were 3 g/L of NaNO 3,30 g/L of sucrose,3 g/L of yeast powder and 7.0 of initial pH value.Under this condition,the degradation rate of β-carotene could reach 93.35%.After being treated by strain HC-3,the eight flavor components of dihydroactyl lactone, 4,7,9-Mega three ene-3- keton, 4- hydroxyl- β-two hydrogen damascene,9-hydroxy-4,7-Mega three ene ketene,triethyl citrate, 4- (3-hydroxyl butyl)-3,5,5- three methyl-2- cyclohexene-1- ketone,tetradecanoic acid and neophytadiene in the reconstituted tobacco concentrate were significantly increased.Compared with the untreated reconstituted tobacco concentrate,the fragrances made from the reconstituted tobacco concentrate treated by strain HC-3 had better quality,more aroma,higher aroma concentration and lower offensive odor content.
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