JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

离子液体双水相体系结合UPLC检测食醋中杂环胺的方法

李敏,徐东明,杨钰雯,贺姗姗,齐改改

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李敏, 徐东明, 杨钰雯, 等. 离子液体双水相体系结合UPLC检测食醋中杂环胺的方法[J]. 轻工学报, 2022, 37(4): 26-33. doi: 10.12187/2022.04.004
引用本文:李敏, 徐东明, 杨钰雯, 等. 离子液体双水相体系结合UPLC检测食醋中杂环胺的方法[J]. 轻工学报, 2022, 37(4): 26-33.doi:10.12187/2022.04.004
shu
LI Min, XU Dongming, YANG Yuwen, et al. A method for determination of heterocyclic aromatic amines in vinegar by ionic liquid aqueous two-phase extraction coupled with UPLC[J]. Journal of Light Industry, 2022, 37(4): 26-33. doi: 10.12187/2022.04.004
Citation:LI Min, XU Dongming, YANG Yuwen, et al. A method for determination of heterocyclic aromatic amines in vinegar by ionic liquid aqueous two-phase extraction coupled with UPLC[J]. Journal of Light Industry, 2022, 37(4): 26-33.doi:10.12187/2022.04.004
shu

离子液体双水相体系结合UPLC检测食醋中杂环胺的方法

  • 18新利直播 食品与生物工程学院, 河南 郑州 450001

    • 作者简介:李敏(1989—),女,河南省宁陵县人,18新利直播 讲师,博士,主要研究方向为食品中有害物质的检测与控制。E-mail:2017074@zzuli.edu.cn;

  • 基金项目:河南省科技攻关项目(212102310493,222102310463)
    18新利直播 博士科研启动基金项目(13501050065)

  • 中图分类号:TS262.6

A method for determination of heterocyclic aromatic amines in vinegar by ionic liquid aqueous two-phase extraction coupled with UPLC

  • College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

  • Received Date:2021-07-12
    Accepted Date:2022-04-24

    CLC number:TS262.6

  • 摘要:通过单因素试验和正交试验确定了一种离子液体双水相体系的最佳萃取条件,与高效液相色谱(UPLC)技术相结合,建立了一种检测食醋中杂环胺(HAAs)类物质Harman和Norharmane的方法。结果表明,最佳萃取条件为选用[C 6MIM]Br-K 2HPO 4离子液体双水相体系,[C 6MIM]Br用量200 μL,K 2HPO 4用量0.5 g,pH值10,超声时间3 min;在此条件下,Harman和Norharmane的线性范围均为10.0~400.0 μg/mL,相关系数 R 2均为0.999 5, LODs分别为0.50 μg/mL和0.45 μg/mL,精密度、重复性和回收率均良好,即该方法适用于检测食醋中的Harman和Norharmane。
    Abstract:A method of ionic liquid aqueous two-phase system, in which optimum extraction conditions were achieved by both single factor experiment and orthogonal experiment, combined with ultra-high performance liquid chromatography (UPLC), was established to detect Harman and Norharmane in vinegar. The experimental results showed that the optimum extraction conditions were as follows: [C 6MIM]Br-K 2HPO 4were used as the ionic liquid aqueous two-phase system, the amount of [C 6MIM]Br was 200 μL, the amount of K 2HPO 4was 0.5 g, pH value was 10, and the ultrasound time was 3 min. Under the appropriate optimal conditions, both the linear equations of Harman and Norharmane were 10.0~400.0 μg/mL, and both the correlation coefficients R 2were 0.999 5. The limits of detection ( LODs) of Harman and Norharmane were 0.50 μg/mL and 0.45 μg/mL, respectively. All the precision experiment, repeatability experiment, and recovery experiment showed that the method had better accuracy and precision. That is, the method could be used for the determination of Harman and Norharmane in vinegar.
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    李敏, 徐东明, 杨钰雯, 等. 离子液体双水相体系结合UPLC检测食醋中杂环胺的方法[J]. 轻工学报, 2022, 37(4): 26-33. doi: 10.12187/2022.04.004
    引用本文:李敏, 徐东明, 杨钰雯, 等. 离子液体双水相体系结合UPLC检测食醋中杂环胺的方法[J]. 轻工学报, 2022, 37(4): 26-33.doi:10.12187/2022.04.004
    shu
    LI Min, XU Dongming, YANG Yuwen, et al. A method for determination of heterocyclic aromatic amines in vinegar by ionic liquid aqueous two-phase extraction coupled with UPLC[J]. Journal of Light Industry, 2022, 37(4): 26-33. doi: 10.12187/2022.04.004
    Citation:LI Min, XU Dongming, YANG Yuwen, et al. A method for determination of heterocyclic aromatic amines in vinegar by ionic liquid aqueous two-phase extraction coupled with UPLC[J]. Journal of Light Industry, 2022, 37(4): 26-33.doi:10.12187/2022.04.004
    shu

    离子液体双水相体系结合UPLC检测食醋中杂环胺的方法

      作者简介:李敏(1989—),女,河南省宁陵县人,18新利直播 讲师,博士,主要研究方向为食品中有害物质的检测与控制。E-mail:2017074@zzuli.edu.cn
    • 18新利直播 食品与生物工程学院, 河南 郑州 450001
    • 收稿日期: 2021-07-12
    • 录用日期: 2022-04-24
    基金项目:河南省科技攻关项目(212102310493,222102310463)18新利直播 博士科研启动基金项目(13501050065)

    摘要:通过单因素试验和正交试验确定了一种离子液体双水相体系的最佳萃取条件,与高效液相色谱(UPLC)技术相结合,建立了一种检测食醋中杂环胺(HAAs)类物质Harman和Norharmane的方法。结果表明,最佳萃取条件为选用[C6MIM]Br-K2HPO4离子液体双水相体系,[C6MIM]Br用量200 μL,K2HPO4用量0.5 g,pH值10,超声时间3 min;在此条件下,Harman和Norharmane的线性范围均为10.0~400.0 μg/mL,相关系数R2均为0.999 5,LODs分别为0.50 μg/mL和0.45 μg/mL,精密度、重复性和回收率均良好,即该方法适用于检测食醋中的Harman和Norharmane。

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