JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

微高压CO2对Pseudomonas deceptionensisCM2的杀菌效应研究

张艺林,刘静飞,任梦梦,牛力源,张志坚

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张艺林, 刘静飞, 任梦梦, 等. 微高压CO2对Pseudomonas deceptionensis CM2的杀菌效应研究[J]. 轻工学报, 2022, 37(5): 24-31. doi: 10.12187/2022.05.003
引用本文:张艺林, 刘静飞, 任梦梦, 等. 微高压CO2对Pseudomonas deceptionensis CM2的杀菌效应研究[J]. 轻工学报, 2022, 37(5): 24-31.doi:10.12187/2022.05.003
shu
ZHANG Yilin, LIU Jingfei, REN Mengmeng, et al. The bactericidal effect of petit-high pressure CO2 on Pseudomonas deceptionensis CM2[J]. Journal of Light Industry, 2022, 37(5): 24-31. doi: 10.12187/2022.05.003
Citation:ZHANG Yilin, LIU Jingfei, REN Mengmeng, et al. The bactericidal effect of petit-high pressure CO2 on Pseudomonas deceptionensis CM2[J]. Journal of Light Industry, 2022, 37(5): 24-31.doi:10.12187/2022.05.003
shu

微高压CO2对Pseudomonas deceptionensisCM2的杀菌效应研究

  • 1. 18新利直播 食品与生物工程学院, 河南 郑州 450001;

  • 2. 河南省冷链食品质量安全控制重点实验室, 河南 郑州 450001;

  • 3. 食品生产与安全河南省协同创新中心, 河南 郑州 450001

    • 作者简介:张艺林(1996-),女,河南省南阳市人,18新利直播 硕士研究生,主要研究方向为食品加工与安全控制。E-mail:18567159787@163.com;

  • 基金项目:国家自然科学基金项目(32001801)
    18新利直播 博士科研基金资助项目(13501050059)
    大学生创新创业训练计划项目(S202110462055)

  • 中图分类号:TS201.1

The bactericidal effect ofpetit-high pressure CO2onPseudomonas deceptionensisCM2

  • 1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;

  • 2. He'nan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou 450001, China;

  • 3. Collaborative Innovation Center of Food Production and Safety of He'nan Province, Zhengzhou 450001, China

  • Received Date:2021-10-21
    Accepted Date:2022-03-21

    CLC number:TS201.1

  • 摘要:以分离自腐败鸡肉的 Pseudomonas deceptionensisCM2为对象,研究微高压CO 2( p-HPCD)杀菌技术在不同压力、保压时间下对 P.deceptionensisCM2的杀菌效果。结果表明: p-HPCD能够有效杀灭 P.deceptionensisCM2,且杀菌效果随CO 2压力的增加和保压时间的延长而逐渐增强;经 p-HPCD在1.3 MPa、25 ℃条件下分别处理2 h、4 h和6 h后, P.deceptionensisCM2活菌数分别降低1.48 log 10CFU/mL、3.11 log 10CFU/mL和3.55 log 10CFU/mL;经 p-HPCD在1.3 MPa、25 ℃条件下处理4 h后, P.deceptionensisCM2细胞表面出现明显皱缩和裂痕,胞外核酸和蛋白发生渗漏,细胞膜通透性和细胞膜电位也发生显著改变。
    Abstract:This paper aimed to investigate the bactericidal effect of petit-high pressure carbon dioxide ( p-HPCD) against Pseudomonas deceptionensisCM2 isolated from chicken as a function of CO 2pressure or process time. The results showed that the bactericidal effect of p-HPCD on P.deceptionensisCM2 gradually enhanced with increasing CO 2pressure or prolonging process time. After p-HPCD treatment under 1.3 MPa at 25 ℃ for 2 h, 4 h and 6 h, the populations of P.deceptionensisCM2 were decreased by 1.48 log 10CFU/mL, 3.11 log 10CFU/mL and 3.55 log 10CFU/mL, respectively. p-HPCD treatment under 1.3 MPa at 25 ℃ for 4 h resulted in obvious shrinkage and cracks on the surface of P.deceptionensisCM2 cells. Moreover, p-HPCD treatment caused the leakage of nucleic acids and proteins from P.deceptionensisCM2 cells. Cell membrane permeability and membrane potential also significantly changed after p-HPCD treatment.
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    张艺林, 刘静飞, 任梦梦, 等. 微高压CO2对Pseudomonas deceptionensis CM2的杀菌效应研究[J]. 轻工学报, 2022, 37(5): 24-31. doi: 10.12187/2022.05.003
    引用本文:张艺林, 刘静飞, 任梦梦, 等. 微高压CO2对Pseudomonas deceptionensis CM2的杀菌效应研究[J]. 轻工学报, 2022, 37(5): 24-31.doi:10.12187/2022.05.003
    shu
    ZHANG Yilin, LIU Jingfei, REN Mengmeng, et al. The bactericidal effect of petit-high pressure CO2 on Pseudomonas deceptionensis CM2[J]. Journal of Light Industry, 2022, 37(5): 24-31. doi: 10.12187/2022.05.003
    Citation:ZHANG Yilin, LIU Jingfei, REN Mengmeng, et al. The bactericidal effect of petit-high pressure CO2 on Pseudomonas deceptionensis CM2[J]. Journal of Light Industry, 2022, 37(5): 24-31.doi:10.12187/2022.05.003
    shu

    微高压CO2对Pseudomonas deceptionensisCM2的杀菌效应研究

      作者简介:张艺林(1996-),女,河南省南阳市人,18新利直播 硕士研究生,主要研究方向为食品加工与安全控制。E-mail:18567159787@163.com
    • 1. 18新利直播 食品与生物工程学院, 河南 郑州 450001;
    • 2. 河南省冷链食品质量安全控制重点实验室, 河南 郑州 450001;
    • 3. 食品生产与安全河南省协同创新中心, 河南 郑州 450001
    • 收稿日期: 2021-10-21
    • 录用日期: 2022-03-21
    基金项目:国家自然科学基金项目(32001801)18新利直播 博士科研基金资助项目(13501050059)大学生创新创业训练计划项目(S202110462055)

    摘要:以分离自腐败鸡肉的Pseudomonas deceptionensisCM2为对象,研究微高压CO2(p-HPCD)杀菌技术在不同压力、保压时间下对P.deceptionensisCM2的杀菌效果。结果表明:p-HPCD能够有效杀灭P.deceptionensisCM2,且杀菌效果随CO2压力的增加和保压时间的延长而逐渐增强;经p-HPCD在1.3 MPa、25 ℃条件下分别处理2 h、4 h和6 h后,P.deceptionensisCM2活菌数分别降低1.48 log10CFU/mL、3.11 log10CFU/mL和3.55 log10CFU/mL;经p-HPCD在1.3 MPa、25 ℃条件下处理4 h后,P.deceptionensisCM2细胞表面出现明显皱缩和裂痕,胞外核酸和蛋白发生渗漏,细胞膜通透性和细胞膜电位也发生显著改变。

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