JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

超声波辅助碱提类PSE鸡肉分离蛋白-EGCG复合体系的理化特性

李可,王琳梦,高慧健,梁滢钰,杜曼婷,吴楠,王昱,白艳红

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李可, 王琳梦, 高慧健, 等. 超声波辅助碱提类PSE鸡肉分离蛋白-EGCG复合体系的理化特性[J]. 轻工学报, 2023, 38(2): 14-23,62. doi: 10.12187/2023.02.002
引用本文:李可, 王琳梦, 高慧健, 等. 超声波辅助碱提类PSE鸡肉分离蛋白-EGCG复合体系的理化特性[J]. 轻工学报, 2023, 38(2): 14-23,62.doi:10.12187/2023.02.002
shu
LI Ke, WANG Linmeng, GAO Huijian, et al. Physicochemical properties of ultrasound-assisted alkali extraction treated PSE-like chicken protein isolate-EGCG composite system[J]. Journal of Light Industry, 2023, 38(2): 14-23,62. doi: 10.12187/2023.02.002
Citation:LI Ke, WANG Linmeng, GAO Huijian, et al. Physicochemical properties of ultrasound-assisted alkali extraction treated PSE-like chicken protein isolate-EGCG composite system[J]. Journal of Light Industry, 2023, 38(2): 14-23,62.doi:10.12187/2023.02.002
shu

超声波辅助碱提类PSE鸡肉分离蛋白-EGCG复合体系的理化特性

  • 18新利直播 食品与生物工程学院/河南省冷链食品质量安全控制重点实验室/中原食品实验室/食品生产与安全河南省协同创新中心, 河南 郑州 450001

    • 作者简介:李可(1986-),男,山东省济南市人,18新利直播 副教授,博士,主要研究方向为肉制品加工与质量控制。E-mail:like@zzuli.edu.cn;

  • 基金项目:国家自然科学基金面上项目(32072243)；河南省优秀青年科学基金项目(222300420092)；河南省大学生创新创业训练计划项目(202210462071)；18新利直播 大学生科技活动项目(202101)

  • 中图分类号:TS251.1

Physicochemical properties of ultrasound-assisted alkali extraction treated PSE-like chicken protein isolate-EGCG composite system

  • College of Food and Bioengineering/Henan Key Laboratory of Cold Chain Food Quality and Safety Control/Food Laboratory of Zhongyuan/Collaborative Innovation Center of Food Production and Safety of Henan Province, Zhengzhou University of Light Industry, Zhengzhou 450001, China

  • Received Date:2022-10-09

    CLC number:TS251.1

  • 摘要:为了提高类PSE(Pale,Soft and Exudative)鸡肉分离蛋白的加工利用,防止表没食子儿茶素没食子酸酯(EGCG)的氧化降解,分析了碱溶酸沉法和超声波(20 kHz,450 W,10 min)辅助碱提法(UAE)对类PSE鸡肉分离蛋白结构的影响及其对EGCG的保护作用。结果表明:与碱溶酸沉处理相比,经UAE提取的类PSE鸡肉分离蛋白的平均粒径和浊度减小、Zeta电位绝对值增加,添加EGCG后,复合体系的平均粒径明显减小( P＜0.05);经UAE提取的类PSE鸡肉分离蛋白的 α-螺旋与 β-折叠相对含量分别下降至12.84%和11.96%, β-转角相对含量上升至63.17%( P＜0.05),添加EGCG后 α-螺旋相对含量下降至9.07%, β-折叠相对含量上升至19.08%( P＜0.05);经UAE提取的类PSE鸡肉分离蛋白与EGCG之间具有更强的结合能力,结合位点较大(1.265),对EGCG的保护效果更好。UAE改变了类PSE鸡肉分离蛋白的结构,增强了类PSE鸡肉分离蛋白与EGCG的相互作用,减缓了EGCG被氧化降解。
    Abstract:In order to improve the utilization of PSE (Pale, Soft and Exudative)-like chicken protein isolate and to prevent the oxidative degradation of epigallocatechin-3-gallate (EGCG), the effects of alkali solubilization andextraction followed by acid depositionprecipitation method and ultrasound (20 kHz, 450 W, 10 min) assisted alkali methodextraction (UAE) on the structure of PSE-like chicken protein isolate and their protection on EGCG were analyzed. The results showed that the average particle size and turbidity of PSE-like chicken protein isolate obtained by UAE decreased and the absolute value of the potential increased compared to the alkali extraction followed by acid precipitation treatment, the average particle size of the composite system was significantly decreased after the addition of EGCG (Pα-helix and β-sheet of PSE-like chicken protein isolate by UAE-treated were decreased to 12.84% and 11.96%, respectively, while the relative content of β-turn was increased to 63.17% (Pα-helix decreased significantly to 9.07% and the relative content of β-sheet increased significantly to 19.08% after the addition of EGCG (P<0.05). The PSE-like chicken protein isolate obtained by UAE had the stronger binding ability to EGCG, with a larger binding site (1.265), and better protective effect on EGCG. UAE altered the structure of PSE-like chicken protein isolate, enhanced the interaction of PSE chicken protein isolate with EGCG, and slowed down the oxidative degradation of EGCG.
    1. [1]

      ZHAO X,CHEN X,HAN M Y,et al.Application of isoelectric solubilization/precipitation processing to improve gelation properties of protein isolated from pale, soft, exudative (PSE)-like chicken breast meat[J].LWT-Food Science and Technology,2016,72:141-148.

    2. [2]

      DONG M,CHEN H Q,ZHANG Y M,et al.Processing properties and improvement of pale,soft,and exudative-like chicken meat:A review[J].Food and Bioprocess Technology,2020,13(8):1280-1291.

    3. [3]

      YANG Z,SUN J,LI Z,et al.Robustness of protein:Using pH shifting and low speed shearing to partially recover conformation and dispersibility of myosin from pale,soft,exudative (PSE)-like chicken breast[J].LWT-Food Science and Technology,2021,138:110786.

    4. [4]

      RAHMAN M M,LAMSAL B P.Ultrasound-assisted extraction and modification of plant-based proteins:Impact on physicochemical, functional, and nutritional properties[J].Comprehensive Reviews in Food Science and Food Safety,2021,20(2):1457-1480.

    5. [5]

      李可,李三影,张瑞,等.超声辅助碱法提取对类PSE鸡肉蛋白提取率和功能特性的影响[J].肉类研究,2021,35(10):1-7.

    6. [6]

      薛丽莹,田泽鹏,陈若桐,等.pH处理对EGCG与大豆蛋白相互作用的影响[J].食品科技,2022,47(7):121-128.

    7. [7]

      HAN X,LIANG Z Q,TIAN S F,et al.Epigallocatechin gallate (EGCG) modification of structural and functional properties of whey protein isolate[J].Food Research International,2022,158:111534.

    8. [8]

      CHEN W J,WANG H Y,WANG W J,et al.Binding affinity and antioxidant activity of the complex of (-)-epigallocatechin-3-gallate and whey protein isolate:Effect of ultrasound pretreatment[J].Journal of Food Process Engineering,2020,43(5):e13081.

    9. [9]

      闫世长,徐静雯,武利春,等.超声复合碱处理对大豆分离蛋白乳化性影响及其对表没食子儿茶素没食子酸酯保护作用[J].食品科学,2021,42(7):134-141.

    10. [10]

      LI K,CHEN L,ZHAO Y Y,et al.A comparative study of chemical composition,color,and thermal gelling properties of normal and PSE-like chicken breast meat[J].CyTA-Journal of Food,2015,13(2):213-219.

    11. [11]

      李可,李三影,扶磊,等.低频高强度超声波对鸡胸肉肌原纤维蛋白性质的影响[J].食品科学,2020,41(23):122-129.

    12. [12]

      代世成,连子腾,马林智,等.超声预处理对大豆分离蛋白-儿茶素非共价/共价复合物结构及功能的影响[J].食品科学,2022,43(1):102-110.

    13. [13]

      ZHAO X,BAI Y,XING T,et al.Use of an isoelectric solubilization/precipitation process to modify the functional properties of PSE(pale, soft, exudative)-like chicken meat protein:a mechanistic approach[J].Food Chemistry,2018,248:201-209.

    14. [14]

      JAMBRAK A R,MASON T J,LELAS V,et al.Effect of ultrasound treatment on particle size and molecular weight of whey proteins[J].Journal of Food Engineering,2014,121:15-23.

    15. [15]

      CHEN J,ZHANG X,CHEN X,et al.Phenolic modification of myofibrillar protein enhanced by ultrasound:The structure of phenol matters[J].Food Chemistry,2022,386:132662.

    16. [16]

      KANG S H,ZHANG J,GUO X B,et al.Effects of ultrasonic treatment on the structure,functional properties of chickpea protein isolate and its digestibility in vitro[J].Foods,2022,11(6):880.

    17. [17]

      李杨,闫世长,徐静雯,等.超声复合碱处理大豆蛋白与EGCG复合物功能特性研究[J].农业机械学报,2021,52(2):364-370.

    18. [18]

      LIU C Z,LYU N,REN G R,et al.Explore the interaction mechanism between zein and EGCG using multi-spectroscopy and molecular dynamics simulation methods[J].Food Hydrocolloids,2021,120:106906.

    19. [19]

      殷春燕,昝立峰,邢浩春,等.超声辅助提取对蛋白质的功能性质和结构影响的研究进展[J].食品科技,2022,47(1):246-253.

    20. [20]

      SHANG W H,YAN J N,DU Y N,et al.Functional properties of gonad protein isolates from three species of sea urchin:A comparative study[J].Journal of Food Science,2020,85(11):3679-3689.

    21. [21]

      XU Q D,YU Z L,ZENG W C.Structural and functional modifications of myofibrillar protein by natural phenolic compounds and their application in pork meatball[J].Food Research International,2021,148:110593.

    22. [22]

      岳鑫,包怡红.基于荧光及紫外光谱法对红松种鳞多酚与乳清蛋白相互作用的研究[J].现代食品科技,2019,35(7):114-120.

    23. [23]

      刘勤勤,朱科学,郭晓娜,等.茶多酚与大豆分离蛋白的相互作用[J].食品科学,2015,36(17):43-47.

    24. [24]

      文鹏程,焦瑶瑶,张卫兵,等.茶多酚对牛奶蛋白结构的影响[J].食品与发酵工业,2020,46(8):40-47.

    25. [25]

      李庆舒,程琳,邓红,等.三种多酚与牛血清蛋白相互作用的初步研究[J].食品与发酵工业,2020,46(3):180-187.

    26. [26]

      张秋会,黄现青,李苗云,等.傅里叶红外光谱法研究肌肉蛋白质的二级结构[J].食品与发酵工业,2015,41(10):247-251.

    27. [27]

      YAN S Z,XU J W,ZHANG X Y,et al.Effect of pH-shifting treatment on the structural and functional properties of soybean protein isolate and its interactions with (-)-epigallocatechin-3-gallate[J].Process Biochemistry,2021,101:190-198.

    28. [28]

      CHEN W J,WANG W J,MA X B,et al.Effect of pH-shifting treatment on structural and functional properties of whey protein isolate and its interaction with (-)-epigallo-catechin-3-gallate[J].Food Chemistry,2018,274:234-241.

    29. [29]

      HE Z Y,CHEN J,MOSER S E,et al.Interaction of beta-casein with (-)-epigallocatechin-3-gallate assayed by fluorescence quenching:Effect of thermal processing temperature[J].International Journal of Food Science and Technology,2016,51(2):342-348.

    30. [30]

      BOHIN M C,VINCKEN J P,VAN DER HIJDEN H T W M,et al.Efficacy of food proteins as carriers for flavonoids[J].Journal of Agricultural and Food Chemistry,2012,60(16):4136-4143.

    31. [31]

      BAE M J,ISHII T,MINODA K,et al.Albumin stabilizes (-)-epigallocatechin gallate in human serum:Binding capacity and antioxidant property[J].Molecular Nutrition & Food Research,2009,53(6):709-715.

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    李可, 王琳梦, 高慧健, 等. 超声波辅助碱提类PSE鸡肉分离蛋白-EGCG复合体系的理化特性[J]. 轻工学报, 2023, 38(2): 14-23,62. doi: 10.12187/2023.02.002
    引用本文:李可, 王琳梦, 高慧健, 等. 超声波辅助碱提类PSE鸡肉分离蛋白-EGCG复合体系的理化特性[J]. 轻工学报, 2023, 38(2): 14-23,62.doi:10.12187/2023.02.002
    shu
    LI Ke, WANG Linmeng, GAO Huijian, et al. Physicochemical properties of ultrasound-assisted alkali extraction treated PSE-like chicken protein isolate-EGCG composite system[J]. Journal of Light Industry, 2023, 38(2): 14-23,62. doi: 10.12187/2023.02.002
    Citation:LI Ke, WANG Linmeng, GAO Huijian, et al. Physicochemical properties of ultrasound-assisted alkali extraction treated PSE-like chicken protein isolate-EGCG composite system[J]. Journal of Light Industry, 2023, 38(2): 14-23,62.doi:10.12187/2023.02.002
    shu

    超声波辅助碱提类PSE鸡肉分离蛋白-EGCG复合体系的理化特性

      作者简介:李可(1986-),男,山东省济南市人,18新利直播 副教授,博士,主要研究方向为肉制品加工与质量控制。E-mail:like@zzuli.edu.cn
    • 18新利直播 食品与生物工程学院/河南省冷链食品质量安全控制重点实验室/中原食品实验室/食品生产与安全河南省协同创新中心, 河南 郑州 450001
    • 收稿日期: 2022-10-09
    基金项目:国家自然科学基金面上项目(32072243)；河南省优秀青年科学基金项目(222300420092)；河南省大学生创新创业训练计划项目(202210462071)；18新利直播 大学生科技活动项目(202101)

    摘要:为了提高类PSE(Pale,Soft and Exudative)鸡肉分离蛋白的加工利用,防止表没食子儿茶素没食子酸酯(EGCG)的氧化降解,分析了碱溶酸沉法和超声波(20 kHz,450 W,10 min)辅助碱提法(UAE)对类PSE鸡肉分离蛋白结构的影响及其对EGCG的保护作用。结果表明:与碱溶酸沉处理相比,经UAE提取的类PSE鸡肉分离蛋白的平均粒径和浊度减小、Zeta电位绝对值增加,添加EGCG后,复合体系的平均粒径明显减小(P＜0.05);经UAE提取的类PSE鸡肉分离蛋白的α-螺旋与β-折叠相对含量分别下降至12.84%和11.96%,β-转角相对含量上升至63.17%(P＜0.05),添加EGCG后α-螺旋相对含量下降至9.07%,β-折叠相对含量上升至19.08%(P＜0.05);经UAE提取的类PSE鸡肉分离蛋白与EGCG之间具有更强的结合能力,结合位点较大(1.265),对EGCG的保护效果更好。UAE改变了类PSE鸡肉分离蛋白的结构,增强了类PSE鸡肉分离蛋白与EGCG的相互作用,减缓了EGCG被氧化降解。

    English Abstract

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