红烧乳鸽中11种极性杂环胺的UHPLC-Q-Orbitrap HRMS检测

1. 仲恺农业工程学院轻工食品学院, 广东广州 510225;
2. 广东省岭南特色食品科学与技术重点实验室, 广东广州 510225;
3. 农业农村部岭南特色食品绿色加工与智能制造重点实验室, 广东广州 510225;
4. 广州质量监督检测研究院, 广东广州 510110;
5. 梅州市金绿现代农业发展有限公司, 广东梅州 514000

作者简介:叶惠萍(1997-),女,广东省东莞市人,仲恺农业工程学院硕士研究生,主要研究方向为食品加工与安全。E-mail:hpye@foxmail.com;

基金项目:国家自然科学基金青年基金项目(32001729)；广东省普通高校特色创新项目(2022KTSCX058)；广东省岭南特色食品科学与技术重点实验室项目(2021B1212040013)；广东省驻镇帮镇扶村农村科技特派员项目(KTP20210224)；梅州市兴宁市肉鸽产业园技术研发项目(粤农农函〔2021〕1024号)；广东省仲恺农业工程学院-广州质量监督检测研究院联合培养研究生示范基地项目(粤教研函〔2021〕2号)；仲恺农业工程学院研究生科技创新基金项目(KJCX2022013)
中图分类号:TS201.6

Determination of 11 polar heterocyclic aromatic amines in braised squab By UHPLC-Q-Orbitrap HRMS

1. College of Light Industry and Food Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
2. Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou 510225, China;
3. Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture, Guangzhou 510225, China;
4. Guangzhou Quality Supervision and Testing Institute, Guangzhou 510110, China;
5. Meizhou Jinlv Modern Agriculture Development Co., Ltd., Meizhou 514000, China
Received Date:2022-05-04

CLC number:TS201.6

摘要:以市售红烧乳鸽为研究对象,建立超高效液相色谱-四极轨道高分辨质谱(UHPLC-Q-Orbitrap HRMS)联用法,并对红烧乳鸽样品中不同部位11种极性杂环胺含量进行检测。结果表明:建立的方法线性关系良好、灵敏度较高,11种杂环胺的质量准确度均＜3×10 ^-6,决定系数 R ²≥0.997 2,方法的检出限为0.03~0.60 μg/kg,定量限为0.10~2.00 μg/kg;该方法具有稳定的回收率和一定的精确性,11种杂环胺的回收率为83.40%~114.80%,精密度为1.40%~7.20%,结果良好;红烧乳鸽中主要检出MeIQx(0.10~0.46 μg/kg)、PhIP(0.10~0.53 μg/kg)和DMIP(0.10~0.25 μg/kg)3种杂环胺,乳鸽部位中HAAs总含量由多到少依次为鸽跟>鸽胸>鸽翅>鸽腿。
- 杂环胺/
- 红烧乳鸽/
- 超高效液相色谱-四极轨道高分辨质谱联用法
Abstract:With commericially available braised squab as research object, UHPLC-Q-Orbitrap HRMS was established. Eleven polar heterocyclic aromatic amines in braised squab were detected by UHPLC-Q-Orbitrap HRMS. The results showed that the method had good linearity and high mass accuracy. The mass accuracy of 11 heterocyclic aromatic amines was <3×10 ^-6. The determination coefficients were greater than 0.997 2. The limit of detection was 0.03~0.60 μg/kg, and the limit of quantification was 0.10~2.00 μg/kg. This method had stable recovery and certain accuracy. The recovery of 11 heterocyclic aromatic amines at 3 spiked concentrations ranged from 83.40% to 114.80%, and the precision ranged from 1.40% to 7.20%. The main heterocyclic aromatic amines detected in braised squab were MeIQx (0.10~0.46 μg/kg), PhIP (0.10~0.53 μg/kg) and DMIP (0.10~0.25 μg/kg). The total content of heterocyclic aromatic amines in 4 parts of braised squab was the heel, breast, wing and leg in descending oder.
- heterocyclic aromatic amines/
- braised squab/
- UHPLC-Quadrupole-Orbitrap high resolution mass spectrometry
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沈阳化工大学材料科学与工程学院沈阳 110142

红烧乳鸽中11种极性杂环胺的UHPLC-Q-Orbitrap HRMS检测

作者简介:叶惠萍(1997-),女,广东省东莞市人,仲恺农业工程学院硕士研究生,主要研究方向为食品加工与安全。E-mail:hpye@foxmail.com;

Determination of 11 polar heterocyclic aromatic amines in braised squab By UHPLC-Q-Orbitrap HRMS

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通讯作者:陈斌, bchen63@163.com

红烧乳鸽中11种极性杂环胺的UHPLC-Q-Orbitrap HRMS检测

作者简介:叶惠萍(1997-),女,广东省东莞市人,仲恺农业工程学院硕士研究生,主要研究方向为食品加工与安全。E-mail:hpye@foxmail.com

English Abstract

Determination of 11 polar heterocyclic aromatic amines in braised squab By UHPLC-Q-Orbitrap HRMS

目录

红烧乳鸽中11种极性杂环胺的UHPLC-Q-Orbitrap HRMS检测

作者简介:叶惠萍(1997-),女,广东省东莞市人,仲恺农业工程学院硕士研究生,主要研究方向为食品加工与安全。E-mail:hpye@foxmail.com;

Determination of 11 polar heterocyclic aromatic amines in braised squab By UHPLC-Q-Orbitrap HRMS

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通讯作者:陈斌, bchen63@163.com

红烧乳鸽中11种极性杂环胺的UHPLC-Q-Orbitrap HRMS检测

作者简介:叶惠萍(1997-),女,广东省东莞市人,仲恺农业工程学院硕士研究生,主要研究方向为食品加工与安全。E-mail:hpye@foxmail.com

English Abstract

Determination of 11 polar heterocyclic aromatic amines in braised squab By UHPLC-Q-Orbitrap HRMS

目录