低温长时间热加工对海参体壁蛋白质消化吸收特性的影响

1. 大连工业大学食品学院/国家海洋食品工程技术研究中心, 辽宁大连 116034;
2. 安徽农业大学茶与食品科技学院, 安徽合肥 230036

作者简介:楚鹏飞(1995-),男,河南省驻马店市人,大连工业大学硕士研究生,主要研究方向为水产品加工过程中品质变化。E-mail:3458003231@qq.com;

基金项目:国家自然科学基金项目（31901615）；大连市高层次人才创新支持计划青年科技之星项目（2021RQ087）
中图分类号:TS254.4

Effect of low-temperature and long-time heat processing on digestion and absorption properties of sea cucumber body wall protein

1. School of Food Science and Technology/National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China;
2. College of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036, China
Received Date:2023-02-28
Accepted Date:2023-04-30

CLC number:TS254.4

摘要:以新鲜海参为原料，于低温（60℃和80℃）条件下进行不同时间（0 h、1 h、3 h、6 h、12 h、24 h和48 h）的热加工，结合体外模拟消化实验和大鼠翻转肠囊模型，对海参体壁蛋白质变化情况（氧化规律、聚集程度、表面疏水性等）进行研究。结果表明：在整个热加工过程中，海参体壁蛋白质的羰基含量逐渐增加，游离巯基含量逐渐减少，蛋白质氧化程度呈时序性加剧；伴随热加工程度的加剧（热加工不足—适度—过度），蛋白质聚集程度先降低后升高再降低，表面疏水性先增强后减弱再增强，蛋白质平均粒径先增大后减小；经体外模拟消化实验和大鼠翻转肠囊模型转运后，蛋白质水解程度、体外消化率和多肽的跨膜转运水平均随加工时间的延长先升高后降低再升高。综上，于60℃条件下热加工3 h时，海参体壁蛋白质的体外消化率最高且吸收特性较好；于80℃条件下加工超过6 h时，有利于提高海参体壁蛋白质的消化吸收特性。
- 低温长时间热加工/
- 海参体壁/
- 蛋白质/
- 体外模拟消化/
- 多肽转运
Abstract:Fresh sea cucumber was processed at low temperature (60℃ and 80℃) for different times (0 h, 1 h, 3 h, 6 h, 12 h, 24 h and 48 h). Combined with simulatior in vitrodigestion and everted-rat-gut-sac model, the changes of protein in the body wall of sea cucumber (SCBWs), including oxidation rule, aggregation degree, surface hydrophobicity, etc, were studied. The results showed that during the whole process of heat processing, the carbonyl content increased gradually, the free sulfhydryl content decreased gradually, and the degree of protein oxidation showed a sequential increase. With the intensification of the degree of heat processing (underworking-moderate-excessive heat processing), the protein aggregation degree first decreased, then increased and then decreased, the surface hydrophobicity first increased and then decreased and then increased, and the mean protein particle size first increased and then decreased. After simulation in vitrodigestion and everted-rat-gut-sac model, the degree of protein hydrolysis, in vitrodigestibility and peptide transport levels were firstly increased, then decreased and then increased with the intensification of heat processing degree. In conclusion, the in vitrodigestibility of SCBWs protein was the highest and the absorption characteristics were better when it was heat processed at 60℃ for 3 h. The digestion and absorption characteristics of SCBWs protein were improved when the thermal treatment time was more than 6 h at 80℃.
- low-temperature and long-time heat processing/
- sea cucumber body wall/
- protein/
- in vitrodigestion simulation/
- polypeptide transport
1. [1]
  李岩.低温加热过程中海参体壁胶原组织结构变化的研究[D].大连:大连工业大学, 2016.
2. [2]
  农业部渔业局.中国渔业统计年鉴[M].北京:中国农业出版社, 2022.
3. [3]
  朱蓓薇.海珍品加工理论与技术的研究[M].北京:科学出版社, 2010.
4. [4]
  LIU Z Q, ZHOU D Y, LIU Y X, et al.Inhibitory effect of natural metal ion chelators on the autolysis of sea cucumber (Stichopus japonicus) and its mechanism[J].Food Research International, 2020, 133:109205.
5. [5]
  YU X L, WEI S B, ZHAO M Y, et al.Effect of heat-treatment times on the microstructure and water absorption properties of sea cucumber[J].International Journal of Food Science and Technology, 2022, 58(2):701-711.
6. [6]
  ZHANG M, LIU Y X, WU Z X, et al.Analysis of texture properties and water-soluble fraction proteome of sea cucumber body wall with different boiling heating treatment[J].Food Chemistry, 2022, 409:13533.
7. [7]
  BHAT Z F, MORTON J D, MASON S L, et al.Cooking does not impair the impact of pulsed electric field on the protein digestion of venison (Cervus elaphus) during in vitro gastrointestinal digestion[J].International Journal of Food Science and Technology, 2021, 56(6):3026-3033.
8. [8]
  温斯颖.不同种类动物肌肉蛋白质消化产物比较研究[D].南京:南京农业大学, 2015.
9. [9]
  BHAT Z F, MORTON J D, BEKHIT A E A, et al.Thermal processing implications on the digestibility of meat, fish and seafood proteins[J].Comprehensive Reviews in Food Science and Food Safety, 2021, 20(5):4511-4548.
10. [10]
  FAN X R, MA Y S, LI M, et al.Thermal treatments and their influence on physicochemical properties of sea cucumbers:A comprehensive review[J].International Journal of Food Science and Technology, 2022, 57(9):5790-5800.
11. [11]
  葛小通, 王红丽, 尹明雨, 等.冷冻即食海参质构特性指标的相关性研究[J].中国食品学报, 2023, 23(1):326-334.
12. [12]
  HE B Y, FENG D D, JIANG D, et al.Studies on quality and physicochemical properties of sea cucumberStichopus japonicusduring low temperature treatment[J].Journal of Chinese Institute of Food Science and Technology, 2019, 19(6):134-140.
13. [13]
  BECKER A, BOULAABA A, PINGEN S, et al.Low temperature cooking of pork meat-physicochemical and sensory aspects[J].Meat Science, 2016, 118:82-88.
14. [14]
  DOMINGUEZ-HEMANDEZ E, SALASEVICIENCE A, ERTBJERG P, et al.Low-temperature long-time cooking of meat:Eating quality and underlying mechanisms[J].Meat Science, 2018, 143:104-113.
15. [15]
  XU Y, FAN H F, ZHAO D L, et al.Optimization of extraction method for water-soluble protein determination by coomassie bright blue method[J].Soybean Science, 2022, 41(2):196-202.
16. [16]
  LIU X X, CHEN Y, CHEN X H, et al.Effect of ginger extract on the oxidation of myofibrillar protein in squid[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(9):225-232.
17. [17]
  许英一, 刘迪, 林巍, 等.热处理改性燕麦蛋白部分性质与表面疏水性的关系[J].食品工业, 2021, 42(8):176-179.
18. [18]
  YU M M, FAN Y C, LIU Y X, et al.Effects of antioxidants of bamboo leaves on protein digestion and transport of cooked abalone muscles[J].Food & Function, 2022, 13(4):1785-1796.
19. [19]
  BHAT Z F, MORTON J D, ZHANG X, et al.Sous-vide cooking improves the quality and in-vitro digestibility of semitendinosus from culled dairy cows[J].Food Research International, 2020, 127:108708.
20. [20]
  DUQUE-ESTRADA P, BERTON-CARABIN C C, NIEUWKOOP M, et al.Protein oxidation and in vitro gastric digestion of processed soy-based matrices[J].Journal of Agricultural & Food Chemistry, 2019, 67(34):9591-9600.
21. [21]
  张旭东, 斯琴其木格, 曾睿, 等.不同热处理条件下羊血浆蛋白体外模拟消化研究[J].食品安全质量检测学报, 2022, 13(18):6049-6056.
22. [22]
  大连工业大学动物实验伦理委员会.SCXK(Liao)2015-0001[S].大连:大连工业大学, 2015.
23. [23]
  阴法文.酪醇脂肪酸酯对贻贝油的抗氧化作用及其吸收代谢机制[D].大连:大连工业大学, 2018.
24. [24]
  JIANG D D, SHEN S K, YU W T, et al.Insights into peptide profiling of sturgeon myofibrillar proteins with low temperature vacuum heating[J].Journal of the Science of Food and Agriculture, 2023, 103(6):2858-2866.
25. [25]
  RIBEIRO A R, BARBAGLIO A, BENEDETTO C D, et al.New insights into mutable collagenous tissue:Correlations between the microstructure and mechanical state of a sea-urchin ligament[J].PLoS One, 2017, 6(9):e24822.
26. [26]
  胡吕霖.烹饪对鲟鱼蛋白质氧化及消化性的影响研究[D].杭州:浙江大学, 2018.
27. [27]
  韩宗元, 邵俊花, 潘燕墨, 等.蛋白质适度加工:热聚集与凝胶品质阐述以及过度聚集的调控对策[J].食品科学, 2023, 44(9):177-184.
28. [28]
  王博, 伊东, 谢梦颖, 等.糖基化大豆分离蛋白对肌原纤维蛋白功能特性的影响[J].食品科学, 2017, 38(7):63-69.
29. [29]
  KAUR L, MAU DENS E, HAISMAN D R, et al.Microstructure and protein digestibility of beef:The effect of cooking conditions as used in stews and curries[J].LWT-Food Science and Technology, 2014, 55(2):612-620.
30. [30]
  DU X J, SUN Y Y, PAN D D, et al.Change of the structure and the digestibility of myofibrillar proteins in Nanjing dry-cured duck during processing[J].Journal of the Science of Food and Agriculture, 2018, 98(8):3140-3147.
31. [31]
  WANG Z Y, WU Z X, ZHAO G H, et al.Effect of air frying and baking on physicochemical properties and digestive properties of scallop (Patinopecten yessoensis) adductor muscle[J].Food Bioscience, 2023, 52:10240.
32. [32]
  ZHOU C Y, PAN D D, SUN Y Y, et al.The effect of cooking temperature on the aggregation and digestion rate of myofibrillar proteins in Jinhua ham[J].Journal of the Science of Food and Agriculture, 2018, 98(9):3563-3570.
33. [33]
  PHILO J S, ARAKAWA T.Mechanisms of protein aggregation[J].Current Pharmaceutical Biotechnology, 2009, 10(4):348-351.
34. [34]
  LIU F J, DONG X P, SHEN S K, et al.Changes in the digestion properties and protein conformation of sturgeon myofibrillar protein treated by low temperature vacuum heating during in vitro digestion[J].Food & Function, 2021, 12(15):6981-6991.
35. [35]
  BORNHORST G M, SINGH R P.Gastric digestion in vivo and in vitro:How the structural aspects of food influence the digestion process[J].Annual Review of Food Science & Technology, 2014, 5(1):111-132.
36. [36]
  丁龙.蛋清肽结构鉴定与完整吸收的构效关系研究[D].长春:吉林大学, 2018.
37. [37]
  SOLADOYE O P, JUAREZ M L, AALHUS J L, et al.Protein oxidation in processed meat:Mechanisms and potential implications on human health[J].Comprehensive Reviews in Food Science and Food Safety, 2015, 14(2):106-122.
1. [1]
  林子璇,董会龙,牛猛,张宾佳,贾才华,许燕,赵思明. 湿碱面加工过程中蛋白质和淀粉的特性变化及淋油静置工艺优化. 轻工学报, 2023, 38(1): 34-44.doi: 10.12187/2023.01.005
2. [2]
  杨开广,张丽华,张玉奎. 蛋白质分离和鉴定的新技术新方法研究进展. 轻工学报, 2012, 27(5): 1-7,12.doi: 10.3969/j.issn.2095-476X.2012.05.001
3. [3]
  吴慈,梁玉,梁振,张丽华,张玉奎. 整体材料的研制及其在蛋白质组色谱分离中的应用. 轻工学报, 2016, 31(4): 1-14.doi: 10.3969/j.issn.2096-1553.2016.4.001
4. [4]
  王晓,吴洲,王宏伟,王榕,陈浩然. 基于深度学习和蛋白质语言模型的抗菌肽预测模型研究. 轻工学报, 2024, 39(2): 12-18.doi: 10.12187/2024.02.002
5. [5]
  方刚. 基于统计语言模型及动态规划算法的蛋白质表达载体的优化设计. 轻工学报, 2016, 31(4): 88-94.doi: 10.3969/j.issn.2096-1553.2016.4.013
6. [6]
  赵建国,李玉,杨德健,朱昌蔼,李刚强. 双酚A废水处理对污泥急性毒性和蛋白质表达的影响. 轻工学报, 2018, 33(6): 19-26.doi: 10.3969/j.issn.2096-1553.2018.06.003
7. [7]
  洪浩洋,杜向阳,颜志刚. 6063-T5铝合金热变形行为及热加工图研究. 轻工学报, 2021, 36(4): 86-96,104.doi: 10.12187/2021.04.011
8. [8]
  李喜宏,杨梦娇,梁富浩,林子沁,李娇,吕芳娥,苗泽,姜瑜倩. 非热加工技术调控果蔬产品内源酶活性研究进展. 轻工学报, 2023, 38(4): 11-19.doi: 10.12187/2023.04.002
9. [9]
  张艳艳,郭朋磊,王文涛,翟娅菲,刘兴丽,张华. 谷朊抗冻多肽对酵母细胞的低温保护作用. 轻工学报, 2023, 38(1): 10-17,26.doi: 10.12187/2023.01.002
10. [10]
  安广杰,胡加松,王章存,赵学伟. 猪骨蛋白酶解制取多肽钙的研究综述. 轻工学报, 2013, 28(6): 27-31.doi: 10.3969/j.issn.2095-476X.2013.06.007
11. [11]
  张肖静,张涵,董永恩,龚瑶瑶,宋亚丽,张杰. 常低温条件下氨氮质量浓度对厌氧消化处理轻工行业废水的影响. 轻工学报, 2024, 39(1): 109-117.doi: 10.12187/2024.01.014
12. [12]
  李松林,林静,蒋长兴,陈晓明,王小花,叶华,王辉. 枸杞多肽的制备及其抗氧化活性研究. 轻工学报, 2016, 31(6): 31-37.doi: 10.3969/j.issn.2096-1553.2016.6.005
13. [13]
  梁楚容,王琴,马路凯,肖更生,刘袆帆. 发酵多肽Asp-Asp-Asp-Tyr和Asp-Tyr-Asp-Asp的稳定性研究. 轻工学报, 2023, 38(2): 48-55.doi: 10.12187/2023.02.006
14. [14]
  张煜,张晓娜,薛桥丽,肖蓉,林奇,胡永金. 扁枝槲寄生提取物体外抗氧化效应研究. 轻工学报, 2016, 31(3): 39-48.doi: 10.3969/j.issn.2096-1553.2016.3.006
15. [15]
  吉杰丽,薛桥丽,谷大海,朱仁俊,胡永金. 普洱茶醇提不同萃取物的体外抑菌活性研究. 轻工学报, 2015, 30(3-4): 15-19.doi: 10.3969/j.issn.2095-476X.2015.3/4.004
16. [16]
  胡海艳,甘祥武,黄秀敏,叶俊豪,谢万勇. 基于易错PCR的β-甘露聚糖酶体外分子定向进化研究. 轻工学报, 2020, 35(4): 8-15.doi: 10.12187/2020.04.002
17. [17]
  朱鹏浩,谢润华,李佳玲,余宁翔,聂小华,孟祥河,陆元超. 香榧油-薯蓣皂素油凝胶的制备及其结构和消化特性研究. 轻工学报, 2024, 39(1): 57-63,117.doi: 10.12187/2024.01.007
18. [18]
  申瑞玲,王英,董吉林. 不同燕麦产品对正常小鼠消化酶及Na⁺-K⁺-ATP酶活性的影响. 轻工学报, 2011, 26(4): 1-5.doi: 10.3969/j.issn.1004-1478.2011.04.001
19. [19]
  朱磊. 大型超市火灾危险性数值模拟. 轻工学报, 2013, 28(3): 86-88.doi: 10.3969/j.issn.2095-476X.2013.03.020
20. [20]
  栗俊广,张旭玥,陈宇豪,王昱,刘骁,白艳红. 鹰嘴豆分离蛋白对猪肉肌原纤维蛋白乳化特性的影响. 轻工学报, 2021, 36(6): 30-37.doi: 10.12187/2021.06.004

点击查看大图

计量

PDF下载量:12
文章访问数:3212
引证文献数:0

通讯作者:陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

低温长时间热加工对海参体壁蛋白质消化吸收特性的影响

作者简介:楚鹏飞(1995-),男,河南省驻马店市人,大连工业大学硕士研究生,主要研究方向为水产品加工过程中品质变化。E-mail:3458003231@qq.com;

Effect of low-temperature and long-time heat processing on digestion and absorption properties of sea cucumber body wall protein

计量

通讯作者:陈斌, bchen63@163.com

低温长时间热加工对海参体壁蛋白质消化吸收特性的影响

作者简介:楚鹏飞(1995-),男,河南省驻马店市人,大连工业大学硕士研究生,主要研究方向为水产品加工过程中品质变化。E-mail:3458003231@qq.com

English Abstract

Effect of low-temperature and long-time heat processing on digestion and absorption properties of sea cucumber body wall protein

目录

低温长时间热加工对海参体壁蛋白质消化吸收特性的影响

作者简介:楚鹏飞(1995-),男,河南省驻马店市人,大连工业大学硕士研究生,主要研究方向为水产品加工过程中品质变化。E-mail:3458003231@qq.com;

Effect of low-temperature and long-time heat processing on digestion and absorption properties of sea cucumber body wall protein

计量

文章相关

通讯作者:陈斌, bchen63@163.com

低温长时间热加工对海参体壁蛋白质消化吸收特性的影响

作者简介:楚鹏飞(1995-),男,河南省驻马店市人,大连工业大学硕士研究生,主要研究方向为水产品加工过程中品质变化。E-mail:3458003231@qq.com

English Abstract

Effect of low-temperature and long-time heat processing on digestion and absorption properties of sea cucumber body wall protein

目录