Abstract:In order to study the effects of different tobacco varieties on the prepared tobacco extracts, Honghuadajinyuan and K326 flue-cured tobacco leaves of the same year, position and grade in Yunlong, Yunnan Province were selected.The tobacco absolute was prepared by supercritical CO ₂fluid extraction technology under the same process conditions.The differences between two tobacco absolutes were analyzed and compared by GC/MS and sensory evaluation in cigarette.The results showed that among the 47 common components identified in the two tobacco absolutes, the top 3 relative contents were nicotine, cibertrienediol and neophydiene. Among the total 15 higher fatty acids and their esters in various types of compounds,the relative contents of K326 tobacco were all higher obviously. In terms of important aroma constituents of tobacco,the relative contents of 10 aroma components including chlorophyll, carotene and cembranoids degradation products were higher in honghuadajinyuan tobacco absolute, while the relative contents of 7 aroma components including phenylalanine degradation products and browning reaction products were higher in K326 tobacco absolute. In the sensory evaluation of cigarette application,the same point of two tobacco absolutes was that they had mainly hay incense and fresh-sweetness aroma, with fruity and green aroma, and the difference was that honghuadajinyuan tobacco absolute could show woody and alcohol-sweetness aroma, while K326 tobacco absolute showed baked, burnt-sweetness and honey sweetness aroma. In terms of virginia tobacco aroma and overall aroma quality,honghuadajinyuan tobacco absolute was relatively better than K326.