加拿大烟叶与云南KRK26烟叶香气成分差异分析
Analysis of differential aroma components between Canada tobacco leaves and Yunnan KRK26 tobacco leaves
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摘要:为了解加拿大烟叶与云南KRK26烟叶的品质区别,通过主成分分析、正交偏最小二乘判别分析等方法,对二者香气成分的差异进行比较分析。结果表明:两种烟叶中共检测出9 类117 种香气成分,加拿大烟叶有114 种,云南KRK26烟叶有113 种,其中110 种为两者共有;筛选得到两种烟叶差异成分65种,占共有香气成分的50.6%,其中加拿大烟叶中22 种成分含量较高,占比为33.8%,主要为半挥发性有机酸类和倍半萜类,43种成分含量较低,占比为66.2%,主要为类胡萝卜素降解产物;相较于云南KRK26烟叶,加拿大烟叶中长链脂肪酸和倍半萜类含量较高,而类胡萝卜素降解产物(如巨豆三烯酮、大马酮、二氢大马酮等)含量相对较低。类胡萝卜素降解产物和半挥发性有机酸的差别,可能是造成两种烟叶香气风格差异的主要因素之一。Abstract:In order to understand the quality difference between Canada and Yunnan KRK26 tobacco leaves, the differences of aroma components were analyzed and compared by main component analysis and orthogonal partial least squares discriminant analysis (OPLS-DA). Test data showed that a total of 117 aroma components belonging to 9 classes were detected in the leaves, of which 110 components were shared by both, while 114 components were present in Canada tobacco leaves and 113 components in KRK26 tobacco leaves. Totally 65 differential aroma components were identified between the two tobacco leaves, accounting for 50.6% of the total aroma components common to them. Furthermore, 22 differential components had higher content (33.8%) in Canada tobacco leaves than that in KRK26 tobacco leaves, whereas 43 metabolites (66.2%) showed the opposite result which are mainly carotenoid degradation products. Compared with KRK26 tobacco leaves, Canada tobacco leaves had higher content of semi-volatile fatty acids and sesquiterpenes, while the content of carotenoid degradation products such as megastigmatrienones, damascenon and dihydrodamascenon was relatively low, which might be one of the factors causing the different sensory aroma characteristics of the two.
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