整体材料的研制及其在蛋白质组色谱分离中的应用

中国科学院分离分析化学重点实验室, 国家色谱研究分析中心, 中国科学院大连化学物理研究所, 辽宁大连 116023
基金项目:国家自然科学基金项目（21575139，21235005，21190043）
中图分类号:O657.7

Preparation of monolithic materials and applications in proteomic chromatographic separation

Key Laborary of Separation Sciences for Analytical Chemistry of Chinese Academy of Sciences, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics of Chinese Academy of Sciences, Dalian 116023, China
Received Date:2016-04-13
Available Online:2016-07-15
CLC number:O657.7

摘要:整体材料由于具有制备简单、传质速度快、低背压、表面易于修饰等优势，在色谱分离领域中应用非常广泛.为使业界了解国内外对整体材料研制的现状，对有机聚合物整体材料、硅胶整体材料、有机-无机杂化整体材料三种整体材料的制备方法及其在蛋白质组学中蛋白质和肽段色谱分离中的应用进行了综述，指出：与填充柱相比，整体柱的分离柱效仍有待进一步提高，可以从整体材料的性能，如比表面积、孔径分布、稳定性、亲水性等方面进行改进.利用整体材料低背压的优势，可制备超长、超细内径毛细管整体柱，有利于微量蛋白质组学样品的高效分离分析.随着对整体材料制备技术的不断深入研究，整体材料也将在食品安全、生命科学、环境问题等众多领域发挥更重要的作用.
- 整体材料/
- 制备方法/
- 色谱分离/
- 蛋白质组学
Abstract:With the advantages of facile preparation,fast mass transfer,low backpressure and easy modification,monolithic materials have been widely used in chromatographic separation.In order to familiarize professionals of home and abroad with the present situation of monolithic materials,this review mainly summarizes the preparation methods of different kinds of monolithic materials (including organic polymer monoliths,silica-based monoliths,organic-inorganic hybrid silica monoliths) and their applications in the separation of proteins or peptides in proteomics.It was pointed out that compared with packed column, monolithic packed column efficiency was to be further improved from the performance of monolithic materials, such as specific surface area, pore size distribution, stablity and hydrophilicity.Taking advantage of the love backpressure of monolithic materials, the caoillary monolithic column with superlong and superfine inner diameter was prepared in order to make high efficient separation analysis of macro proteomics samples. With the further study of monolithic materials preparation technology, monolithic materials will play more important role in the fields of food safety, life science and environment.
- monolithic materials/
- preparation method/
- chromatographic separation/
- proteomics
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沈阳化工大学材料科学与工程学院沈阳 110142

整体材料的研制及其在蛋白质组色谱分离中的应用

Preparation of monolithic materials and applications in proteomic chromatographic separation

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通讯作者:陈斌, bchen63@163.com

整体材料的研制及其在蛋白质组色谱分离中的应用

English Abstract

Preparation of monolithic materials and applications in proteomic chromatographic separation

目录

整体材料的研制及其在蛋白质组色谱分离中的应用

Preparation of monolithic materials and applications in proteomic chromatographic separation

计量

文章相关

通讯作者:陈斌, bchen63@163.com

整体材料的研制及其在蛋白质组色谱分离中的应用

English Abstract

Preparation of monolithic materials and applications in proteomic chromatographic separation

目录