JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

酶解菲律宾蛤仔水煮液制备牛磺酸工艺研究

胡凯伦,高荣春,杨丰盛,刘文转,杨静峰

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胡凯伦, 高荣春, 杨丰盛, 等. 酶解菲律宾蛤仔水煮液制备牛磺酸工艺研究[J]. 轻工学报, 2018, 33(1): 26-33. doi: 10.3969/j.issn.2096-1553.2018.01.004
引用本文:胡凯伦, 高荣春, 杨丰盛, 等. 酶解菲律宾蛤仔水煮液制备牛磺酸工艺研究[J]. 轻工学报, 2018, 33(1): 26-33.doi:10.3969/j.issn.2096-1553.2018.01.004
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HU Kailun, GAO Rongchun, YANG Fengsheng, et al. Study on the preparation of taurine from cooking liquid of Ruditapes philippinarum by enzymatic hydrolysis[J]. Journal of Light Industry, 2018, 33(1): 26-33. doi: 10.3969/j.issn.2096-1553.2018.01.004
Citation:HU Kailun, GAO Rongchun, YANG Fengsheng, et al. Study on the preparation of taurine from cooking liquid of Ruditapes philippinarum by enzymatic hydrolysis[J]. Journal of Light Industry, 2018, 33(1): 26-33.doi:10.3969/j.issn.2096-1553.2018.01.004
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酶解菲律宾蛤仔水煮液制备牛磺酸工艺研究

  • 大连工业大学 食品学院/国家海洋食品工程技术研究中心, 辽宁 大连 116034;

  • 福建省丰盛食品有限公司, 福建 漳浦 363208

    • 通讯作者:杨静峰

  • 基金项目:福建省海洋经济创新发展区域示范项目（2014FJ17）

  • 中图分类号:TS254.9

Study on the preparation of taurine from cooking liquid ofRuditapes philippinarumby enzymatic hydrolysis

  • School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, China;

  • Fujian Fengsheng Food Co., Ltd., Zhangpu 363208, China

    • Corresponding author:YANG Jingfeng,
  • Received Date:2017-11-12
    Available Online:2018-01-15

    CLC number:TS254.9

  • 摘要:研究了酶解菲律宾蛤仔水煮液提取牛磺酸的工艺条件和提高所制备牛磺酸纯度的途径.结果表明：碱性蛋白酶为最适提取酶；在酶解温度45℃，酶解时间3.5 h，酶解pH=8.0的条件下，牛磺酸纯度为8.67%，回收率为95.80%；酶解产物经电渗析除盐后，纯度为21.30%，回收率为89.59%；经阳离子树脂交换后，纯度为31.18%，回收率为83.64%；经乙醇重结晶提纯处理后，纯度达97.28%.
    Abstract:This study aim to explored the preparation and purification technologies of taurine from the cooking liquid of Ruditapes philippinarumusing enzymatic hydrolysis. The results showed that the alkaline protease was the optimum enzyme for extracting taurine. Under the conditions of enzymolysis temperature 45℃, enzymolysis time 3.5 h and enzymolysis pH=8.0, the purity and recovery of taurine reached 8.67% and 95.80%, respectively. After removing salt using electrodialysis, the purity and recovery of taurine reached 21.30% and 89.59%, respectively. After elution on cation exchange resin, the purity and recovery of taurine reached 31.18% and 83.64%, respectively. After the ethanol recrystallization, the purity of taurine reached 97.28%.
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  • 通讯作者:杨静峰,
  • 收稿日期:2017-11-12
  • 刊出日期:2018-01-15
    通讯作者:陈斌, bchen63@163.com
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    胡凯伦, 高荣春, 杨丰盛, 等. 酶解菲律宾蛤仔水煮液制备牛磺酸工艺研究[J]. 轻工学报, 2018, 33(1): 26-33. doi: 10.3969/j.issn.2096-1553.2018.01.004
    引用本文:胡凯伦, 高荣春, 杨丰盛, 等. 酶解菲律宾蛤仔水煮液制备牛磺酸工艺研究[J]. 轻工学报, 2018, 33(1): 26-33.doi:10.3969/j.issn.2096-1553.2018.01.004
    shu
    HU Kailun, GAO Rongchun, YANG Fengsheng, et al. Study on the preparation of taurine from cooking liquid of Ruditapes philippinarum by enzymatic hydrolysis[J]. Journal of Light Industry, 2018, 33(1): 26-33. doi: 10.3969/j.issn.2096-1553.2018.01.004
    Citation:HU Kailun, GAO Rongchun, YANG Fengsheng, et al. Study on the preparation of taurine from cooking liquid of Ruditapes philippinarum by enzymatic hydrolysis[J]. Journal of Light Industry, 2018, 33(1): 26-33.doi:10.3969/j.issn.2096-1553.2018.01.004
    shu

    酶解菲律宾蛤仔水煮液制备牛磺酸工艺研究

      通讯作者:杨静峰
    • 大连工业大学 食品学院/国家海洋食品工程技术研究中心, 辽宁 大连 116034;
    • 福建省丰盛食品有限公司, 福建 漳浦 363208
    • 收稿日期: 2017-11-12
    • 网络出版日期: 2018-01-15
    基金项目:福建省海洋经济创新发展区域示范项目（2014FJ17）

    摘要:研究了酶解菲律宾蛤仔水煮液提取牛磺酸的工艺条件和提高所制备牛磺酸纯度的途径.结果表明：碱性蛋白酶为最适提取酶；在酶解温度45℃，酶解时间3.5 h，酶解pH=8.0的条件下，牛磺酸纯度为8.67%，回收率为95.80%；酶解产物经电渗析除盐后，纯度为21.30%，回收率为89.59%；经阳离子树脂交换后，纯度为31.18%，回收率为83.64%；经乙醇重结晶提纯处理后，纯度达97.28%.

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